PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Giroud, M.* ; Pisani, D.F.* ; Karbiener, M.* ; Barquissau, V.* ; Ghandour, R.A.* ; Tews, D.* ; Fischer-Posovszky, P.* ; Chambard, J.C.* ; Knippschild, U.* ; Niemi, T.* ; Taittonen, M.* ; Nuutila, P.* ; Wabitsch, M.* ; Herzig, S. ; Virtanen, K.A.* ; Langin, D.* ; Scheideler, M. ; Amri, E.Z.*

miR-125b affects mitochondrial biogenesis and impairs brite adipocyte formation and function.

Mol. Metab. 5, 615-25 (2016)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
OBJECTIVE: In rodents and humans, besides brown adipose tissue (BAT), islands of thermogenic adipocytes, termed "brite" (brown-in-white) or beige adipocytes, emerge within white adipose tissue (WAT) after cold exposure or β3-adrenoceptor stimulation, which may protect from obesity and associated diseases. microRNAs are novel modulators of adipose tissue development and function. The purpose of this work was to characterize the role of microRNAs in the control of brite adipocyte formation. METHODS/RESULTS: Using human multipotent adipose derived stem cells, we identified miR-125b-5p as downregulated upon brite adipocyte formation. In humans and rodents, miR-125b-5p expression was lower in BAT than in WAT. In vitro, overexpression and knockdown of miR-125b-5p decreased and increased mitochondrial biogenesis, respectively. In vivo, miR-125b-5p levels were downregulated in subcutaneous WAT and interscapular BAT upon β3-adrenergic receptor stimulation. Injections of an miR-125b-5p mimic and LNA inhibitor directly into WAT inhibited and increased β3-adrenoceptor-mediated induction of UCP1, respectively, and mitochondrial brite adipocyte marker expression and mitochondriogenesis. CONCLUSION: Collectively, our results demonstrate that miR-125b-5p plays an important role in the repression of brite adipocyte function by modulating oxygen consumption and mitochondrial gene expression.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
5.363
1.419
37
39
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Brite Adipocyte ; Mitochondriogenesis ; White Adipocyte ; Mir-125b-5p; Brown Adipose-tissue; In-vivo; Adipogenic Differentiation; Fat Differentiation; Uncoupling Protein; Gene-expression; Microarray Data; Adult Mice; Beige Fat; White
Sprache deutsch
Veröffentlichungsjahr 2016
HGF-Berichtsjahr 2016
ISSN (print) / ISBN 2212-8778
e-ISSN 2212-8778
Zeitschrift Molecular Metabolism
Quellenangaben Band: 5, Heft: 8, Seiten: 615-25 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Amsterdam
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Cancer (IDC)
POF Topic(s) 90000 - German Center for Diabetes Research
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-501900-251
G-501900-252
PubMed ID 27656399
DOI 10.1016/j.molmet.2016.06.005
WOS ID WOS:000386882400003
Scopus ID 84989949578
Erfassungsdatum 2016-10-07
[➜Korrektur melden]