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Kovacs, I. ; Holzmeister, C. ; Wirtz, M.* ; Geerlof, A. ; Fröhlich, T.* ; Römling, G. ; Kuruthukulangarakoola, G.T. ; Linster, E.* ; Hell, R.* ; Arnold, G.J.* ; Durner, J. ; Lindermayr, C.

ROS-mediated inhibition of S-nitrosoglutathione reductase contributes to the activation of anti-oxidative mechanisms.

Front. Plant Sci. 7:1669 (2016)
Verlagsversion Forschungsdaten DOI
Open Access Gold
Creative Commons Lizenzvertrag
Nitric oxide (NO) has emerged as a signaling molecule in plants being involved in diverse physiological processes like germination, root growth, stomata closing and response to biotic and abiotic stress. S-nitrosoglutathione (GSNO) as a biological NO donor has a very important function in NO signaling since it can transfer its NO moiety to other proteins (trans-nitrosylation). Such trans-nitrosylation reactions are equilibrium reactions and depend on GSNO level. The breakdown of GSNO and thus the level of S-nitrosylated proteins are regulated by GSNO-reductase (GSNOR). In this way, this enzyme controls S-nitrosothiol levels and regulates NO signaling. Here we report that Arabiclopsis thahana GSNOR activity is reversibly inhibited by H2O2 in vitro and by paraquat-induced oxidative stress in vivo. Light scattering analyses of reduced and oxidized recombinant GSNOR demonstrated that GSNOR proteins form dimers under both reducing and oxidizing conditions. Moreover, mass spectrometric analyses revealed that H2O2-treatment increased the amount of oxidative modifications on Zn2+-coordinating Cys47 and Cys177. Inhibition of GSNOR results in enhanced levels of S-nitrosothiols followed by accumulation of glutathione. Moreover, transcript levels of redox-regulated genes and activities of glutathione-dependent enzymes are increased in gsnor-ko plants, which may contribute to the enhanced resistance against oxidative stress. In sum, our results demonstrate that reactive oxygen species (ROS)dependent inhibition of GSNOR is playing an important role in activation of anti-oxidative mechanisms to damping oxidative damage and imply a direct crosstalk between ROS- and NO-signaling.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Nitric Oxide ; S-nitrosoglutathione Reductase ; S-nitrosothiols ; Reactive Oxygen Species ; Oxidative Stress ; Hydrogen Peroxide ; Paraquat ; Arabidopsis Thaliana; Dependent Formaldehyde Dehydrogenase; Nitric-oxide Production; Dna-binding Activity; Oxidative Stress; Transcription Factors; Arabidopsis-thaliana; Disease Resistance; Tyrosine Nitration; Posttranslational Modifications; Subcellular-localization
ISSN (print) / ISBN 1664-462X
e-ISSN 1664-462X
Zeitschrift Frontiers in Plant Science
Quellenangaben Band: 7, Heft: , Seiten: , Artikelnummer: 1669 Supplement: ,
Verlag Frontiers
Verlagsort Lausanne
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biochemical Plant Pathology (BIOP)
Institute of Structural Biology (STB)