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Mossina, A. ; Lukas, C. ; Merl-Pham, J. ; Uhl, F.E.* ; Mutze, K. ; Schamberger, A.C. ; Staab-Weijnitz, C.A. ; Jia, J. ; Yildirim, A.Ö. ; Königshoff, M. ; Hauck, S.M. ; Eickelberg, O. ; Meiners, S.

Cigarette smoke alters the secretome of lung epithelial cells.

Proteomics 17:1600243 (2017)
Postprint Forschungsdaten DOI PMC
Open Access Green
Cigarette smoke is the most relevant risk factor for the development of lung cancer and chronic obstructive pulmonary disease (COPD). Many of its more than 4500 chemicals are highly reactive thereby altering protein structure and function. Here, we used subcellular fractionation coupled to label-free quantitative mass spectrometry to globally assess alterations in the proteome of different compartments of lung epithelial cells upon exposure to cigarette smoke extract. Proteomic profiling of the human alveolar-derived cell line A549 revealed the most pronounced changes within the cellular secretome with preferential downregulation of proteins involved in wound healing and extracellular matrix (ECM) organization. In particular, secretion of secreted protein acidic and rich in cysteine (SPARC), a matricellular protein that functions in tissue response to injury, was consistently diminished by cigarette smoke extract in various pulmonary epithelial cell lines and primary cells of human and mouse origin as well as in mouse ex vivo lung tissue cultures. Our study reveals a previously unrecognized acute response of lung epithelial cells to cigarette smoke that includes altered secretion of proteins involved in ECM organization and wound healing. This may contribute to sustained alterations in tissue remodeling as observed in lung cancer and COPD.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Cigarette smoke; Lung; Proteomic profiling; Secretome; Obstructive Pulmonary-disease; Mass-spectrometry; Bronchoalveolar Lavage; Airway Epithelium; Growth-factor; Label-free; Repair; Tissue; Proteome; Sparc
ISSN (print) / ISBN 1615-9853
e-ISSN 1615-9861
Zeitschrift Proteomics
Quellenangaben Band: 17, Heft: 1-2, Seiten: , Artikelnummer: 1600243 Supplement: ,
Verlag Wiley
Verlagsort Hoboken
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Lung Health and Immunity (LHI)
CF Metabolomics & Proteomics (CF-MPC)
German Center for Lung Research (DZL)
Institute of Lung Biology (LHI)