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Azimzadeh, O. ; Scherthan, H.* ; Sarioglu, H. ; Barjaktarovic, Z. ; Conrad, M. ; Vogt, A.* ; Calzada-Wack, J. ; Neff, F. ; Aubele, M. ; Buske, C. ; Atkinson, M.J. ; Tapio, S.

Rapid proteomic remodeling of cardiac tissue caused by total body ionizing radiation.

Proteomics 11, 3299-3311 (2011)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Accidental nuclear scenarios lead to environmental contamination of unknown level. Immediate radiation-induced biological responses that trigger processes leading to adverse health effects decades later are not well understood. A comprehensive proteomic analysis provides a promising means to identify and quantify the initial damage after radiation exposure. Early changes in the cardiac tissue of C57BL/6 mice exposed to total body irradiation were studied, using a dose relevant to both intentional and accidental exposure (3 Gy gamma ray). Heart tissue protein lysates were analyzed 5 and 24 h after the exposure using isotope-coded protein labeling (ICPL) and 2-dimensional difference-in-gel-electrophoresis (2-D DIGE) proteomics approaches. The differentially expressed proteins were identified by LC-ESI-MS-MS. Both techniques showed similar functional groups of proteins to be involved in the initial injury. Pathway analyses indicated that total body irradiation immediately induced biological responses such as inflammation, antioxidative defense, and reorganization of structural proteins. Mitochondrial proteins represented the protein class most sensitive to ionizing radiation. The proteins involved in the initial damage processes map to several functional categories involving cardiotoxicity. This prompts us to propose that these early changes are indicative of the processes that lead to an increased risk of cardiovascular disease after radiation exposure.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Cardiac disease; Cell biology; 2-D DIGE; Ionizing irradiation; Isotope-coded protein labeling; Oxidative stress
ISSN (print) / ISBN 1615-9853
e-ISSN 1615-9861
Zeitschrift Proteomics
Quellenangaben Band: 11, Heft: 6, Seiten: 3299-3311 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Weinheim
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Radiation Biology (ISB)
CF Metabolomics & Proteomics (CF-MPC)
Institute of Developmental Genetics (IDG)
Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
Institute of Pathology (PATH)