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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Use of chemical mutagenesis in mouse embryonic stem cells.
Methods Mol. Biol. 329, 397-407 (2006)
The chemical mutagenesis of mouse embryonic stem (ES) cells is an approach complementary to chemical mutagenesis of spermatogonia in whole animals. It has great potential to contribute significantly to the generation of a comprehensive collection of multiple alleles for most mammalian genes and to facilitate the progression from gene sequence to gene functional analyses. The general strategy includes the treatment of ES cells with a chemical mutagen and the isolation of individual mutagenized clones that are then cultured in duplicate. Whereas one set of samples is cryopreserved for archiving and subsequent generation of germline chimeras by means of blastocyst injection, the second set is used for genotypeor phenotype-driven screens to identify mutant alleles.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Herausgeber
Turksen, K.*
Schlagwörter
Chemical mutagenesis; ES cells; genotype screen; N-ethyl-N-nitrosourea (ENU); methanesulfonic acid ethyl ester (EMS); N-nitroso-N-ethylurea; phenotype screen
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Konferenztitel
Embryonic Stem Cell Protocols : Volume 1: Isolation and Characterization
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 329,
Heft: 27,
Seiten: 397-407
Reihe
Embryonic Stem Cell Protocols
Verlag
Springer
Verlagsort
Berlin [u.a.]
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Experimental Genetics (IEG)