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von Loeffelholz, C.* ; Lieske, S. ; Neuschäfer-Rube, F.* ; Willmes, D.M. ; Raschzok, N.* ; Sauer, I.M.* ; König, J.* ; Fromm, M.* ; Horn, P.* ; Chatzigeorgiou, A. ; Pathe-Neuschäfer-Rube, A.* ; Jordan, J.* ; Pfeiffer, A.F.H.* ; Mingrone, G.* ; Bornstein, S.R. ; Ströhle, P.* ; Harms, C.* ; Wunderlich, F.T.* ; Helfand, S.L.* ; Bernier, M.* ; de Cabo, R.* ; Shulman, G.I.* ; Chavakis, T. ; Püschel, G.P.* ; Birkenfeld, A.L.

The human longevity gene homolog INDY and interleukin-6 interact in hepatic lipid metabolism.

Hepatology 66, 616-630 (2017)
Verlagsversion DOI PMC
Open Access Gold
Reduced expression of the Indy ('I am Not Dead, Yet') gene in lower organisms promotes longevity in a manner akin to caloric restriction. Deletion of the mammalian homolog of Indy (mIndy, Slc13a5) encoding for a plasma membrane associated citrate transporter expressed highly in the liver, protects mice from high-fat diet and aging-induced obesity and hepatic fat accumulation through a mechanism resembling caloric restriction. We aimed to study a possible role of mIndy in human hepatic fat metabolism. In obese, insulin resistant patients with NAFLD, hepatic mIndy expression was increased and mIndy expression was also independently associated with hepatic steatosis. In non-human primates, a two year high fat, high sucrose diet increased hepatic mIndy expression. Liver microarray analysis showed that high mIndy expression was associated with pathways involved in hepatic lipid metabolism and immunological processes. Interleukin-6 (IL-6) was identified as a regulator of mIndy by binding to its cognate receptor. Studies in human primary hepatocytes confirmed that IL-6 markedly induced mIndy transcription via the IL-6-receptor (IL-6R) and activation of the transcription factor Stat3 and a putative start site of the human mIndy promoter was determined. Activation of the IL-6-Stat3 pathway stimulated mIndy expression, enhanced cytoplasmic citrate influx and augmented hepatic lipogenesis in vivo. In contrast, deletion of mIndy completely prevented the stimulating effect of IL-6 on citrate uptake and reduced hepatic lipogenesis. These data show that mIndy is increased in liver of obese humans and non-human primates with NALFD. Moreover, our data identify mIndy as a target gene of IL-6 and determine novel functions of IL-6 via mINDY. Targeting human mINDY may have therapeutic potential in obese patients with NAFLD.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter IL-6; Indy; Insulin Resistance; Liver; NAFLD; Coupled Citrate Transporter; Fatty Liver-disease; Systemic Insulin-resistance; Life-span; Adipose-tissue; Caenorhabditis-elegans; Il-6-deficient Mice; Functional Features; Induced Steatosis; Skeletal-muscle
Sprache englisch
Veröffentlichungsjahr 2017
Prepublished im Jahr 2016
HGF-Berichtsjahr 2016
ISSN (print) / ISBN 0270-9139
e-ISSN 1527-3350
Zeitschrift Hepatology
Quellenangaben Band: 66, Heft: 2, Seiten: 616-630 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Hoboken, NJ
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pancreatic Islet Research (IPI)
POF Topic(s) 90000 - German Center for Diabetes Research
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-502600-001
G-502600-007
G-502600-008
G-502600-012
DOI 10.1002/hep.29089
WOS ID WOS:000405817200028
PubMed ID 28133767
Erfassungsdatum 2016-12-31
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