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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Asymmetrical flow field-flow fractionation of white wine chromophoric colloidal matter.
Anal. Bioanal. Chem. 409, 2757-2766 (2017)
Two analytical separation methods-size-exclusion chromatography and asymmetrical flow field-flow fractionation-were implemented to evaluate the integrity of the colloidal composition of Chardonnay white wine and the impact of pressing and fermentations on the final macromolecular composition. Wine chromophoric colloidal matter, representing UV-visible-absorbing wine macromolecules, was evaluated by optical and structural measurements combined with the description of elution profiles obtained by both separative techniques. The objective of this study was to apply these two types of fractionation on a typical Chardonnay white wine produced in Burgundy and to evaluate how each of them impacted the determination of the macromolecular chromophoric content of wine. UV-visible and fluorescence measurements of collected fractions were successfully applied. An additional proteomic study revealed that grape and microorganism proteins largely impacted the composition of chromophoric colloidal matter of Chardonnay wines. Asymmetrical flow field-flow fractionation appeared to be more reliable and less invasive with respect to the native chemical environment of chromophoric wine macromolecules, and hence is recommended as a tool to fractionate chromophoric colloidal matter in white wines. Graphical Abstract An innovative macromolecular separation method based on Asymmetrical Flow Field-Flow Fractionation was developed to better control colloidal dynamics across Chardonnay white winemaking.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Asymmetrical Flow Field-flow Fractionation ; Chromophoric Colloidal Matter ; White Wine ; Wine Proteins; Size-exclusion Chromatography; Dissolved Organic-matter; Haze-forming Proteins; Red Wines; Maceration Enzymes; Humic Substances; Molecular-weight; Yeast; Polysaccharides; Mannoproteins
ISSN (print) / ISBN
1618-2642
e-ISSN
1618-2650
Zeitschrift
Analytical and Bioanalytical Chemistry
Quellenangaben
Band: 409,
Heft: 10,
Seiten: 2757-2766
Verlag
Springer
Verlagsort
Heidelberg
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Research Unit Analytical BioGeoChemistry (BGC)