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Ozone- and ethylene-induced regulation of a grapevine resveratrol synthase promoter in transgenic tobacco.
Acta Physiol. Plant. 19, 467-474 (1997)
Stilbene synthases (STSs) are enzymes that play a critical role in the biosynthesis of stilbene, phytoalexins in a small number of unrelated plant species, and are induced by various biotic and abiotic stressors like pathogen attack, UV-irradiation or ozone exposure. To investigate the molecular basis for ozone-induced plant stress responses, we have examined the promoter of the grapevine resveratrol synthase (Vst1). In this report we summarize the influence of ozone on gene regulation. In transgenic tobacco a chimeric gene construct, containing the Vst1 promoter combined with the β-glucuronidase (GUS) reporter gene, is rapidly induced by ozone (0.1 µl·l−1, 12 h). The same construct is also strongly induced by ethylene (20 µl·l−1, 12 h). Promoter deletion analysis of the 5′ flanking sequence identified a positive regulatory element between −430 bp and −280 bp. This region contains ethylene-responsive enhancer elements, as well as an elicitor-responsive sequence in inverse orientation.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
elicitor; ethylene; gene regulation; ozone; pathogen; promoter; resveratrol synthase; stilbene synthase
ISSN (print) / ISBN
0137-5881
e-ISSN
1861-1664
Zeitschrift
Acta Physiologiae Plantarum
Quellenangaben
Band: 19,
Heft: 4,
Seiten: 467-474
Verlag
Springer
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Biochemical Plant Pathology (BIOP)