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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Isolation of functional mitochondria from cultured cells and mouse tissues.
Methods Mol. Biol. 1567, 15-32 (2017)
Mitochondria serve as the center stage for a number of cellular processes, including energy production, apoptosis, ion homeostasis, iron and copper processing, steroid metabolism, de novo pyrimidine, and heme biosynthesis. The study of mitochondrial function often requires the purification of intact and respiratory-competent organelles. Here, we provide detailed protocols to isolate functional mitochondria from various types of mammalian cells and mouse tissues, in both crude and pure forms. We introduce the use of nitrogen cavitation for the disruption of plasma membrane and the reproducible isolation of mitochondria-enriched fractions of high yield. Mitochondria that are isolated by these procedures are intact and coupled and can directly be used for several downstream analyses, such as measurements of oxygen consumption and calcium buffering capacity.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Crude Mitochondria ; Mitochondrial Integrity ; Nitrogen Cavitation ; Organelle Isolation ; Pure Mitochondria
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Konferenztitel
Mitochondria
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 1567,
Seiten: 15-32
Verlag
Springer
Verlagsort
Berlin [u.a.]
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Diabetes and Obesity (IDO)