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Seeger, M. ; Karlas, A. ; Soliman, D. ; Pelisek, J.* ; Ntziachristos, V.

Hybrid microscopy of human carotid atheroma by means of optical-resolution optoacoustic and non-linear optical microscopy.

Proc. SPIE 10064:1006455 (2017)
Postprint DOI
Open Access Green
Carotid atheromatosis is causally related to stroke, a leading cause of disability and death. We present the analysis of a human carotid atheroma using a novel hybrid microscopy system that combines optical-resolution optoacoustic (photoacoustic) microscopy and several non-linear optical microscopy modalities (second and third harmonic generation, as well as, two-photon excitation fluorescence) to achieve a multimodal examination of the extracted tissue within the same imaging framework. Our system enables the label-free investigation of atheromatous human carotid tissue with a resolution of about 1 μm and allows for the congruent interrogation of plaque morphology and clinically relevant constituents such as red blood cells, collagen, and elastin. Our data reveal mutual interactions between blood embeddings and connective tissue within the atheroma, offering comprehensive insights into its stage of evolution and severity, and potentially facilitating the further development of diagnostic tools, as well as treatment strategies.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Human carotid atheroma, Collagen, Red blood cells, Multimodal microscopy, Optoacoustic microscopy, Photoacoustic microscopy, Non-linear optical microscopy
ISSN (print) / ISBN 0277-786X
e-ISSN 1996-756X
Konferenztitel Photons Plus Ultrasound: Imaging and Sensing 2017
Konferzenzdatum 28 January 2017
Konferenzort San Francisco, California, United States
Zeitschrift Proceedings of SPIE
Quellenangaben Band: 10064, Heft: , Seiten: , Artikelnummer: 1006455 Supplement: ,
Verlag SPIE
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)