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Schöler, A. ; Jacquiod, S.* ; Vestergaard, G. ; Schulz, S. ; Schloter, M.

Analysis of soil microbial communities based on amplicon sequencing of marker genes.

Biol. Fertil. Soils 53, 485–489 (2017)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The use of cultivation independent methods has revolutionized soil biology in the last decades. Most popular approaches are based on directly extracted DNA from soil and subsequent analysis of PCR-amplified marker genes by next-generation sequencing. While these high-throughput methods offer novel possibilities over cultivation-based approaches, several key points need to be considered to minimize potential biases during library preparation and downstream bioinformatic analysis. This opinion paper highlights crucial steps that should be considered for accurate analysis and data interpretation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Review
Korrespondenzautor
Schlagwörter 16s Rrna ; Amplicon Sequencing ; Bioinformatics ; Next-generation Sequencing ; Sequencing Depth; Pcr Primers; Maize Lines; Diversity; Rhizosphere; Generation; Efficiency
ISSN (print) / ISBN 0178-2762
e-ISSN 1432-0789
Zeitschrift Biology and Fertility of Soils
Quellenangaben Band: 53, Heft: 5, Seiten: 485–489 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort New York
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Comparative Microbiome Analysis (COMI)