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Analysis of soil microbial communities based on amplicon sequencing of marker genes.
Biol. Fertil. Soils 53, 485–489 (2017)
The use of cultivation independent methods has revolutionized soil biology in the last decades. Most popular approaches are based on directly extracted DNA from soil and subsequent analysis of PCR-amplified marker genes by next-generation sequencing. While these high-throughput methods offer novel possibilities over cultivation-based approaches, several key points need to be considered to minimize potential biases during library preparation and downstream bioinformatic analysis. This opinion paper highlights crucial steps that should be considered for accurate analysis and data interpretation.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Times Cited
Scopus
Cited By
Cited By
Altmetric
3.683
1.419
149
164
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Review
Schlagwörter
16s Rrna ; Amplicon Sequencing ; Bioinformatics ; Next-generation Sequencing ; Sequencing Depth; Pcr Primers; Maize Lines; Diversity; Rhizosphere; Generation; Efficiency
Sprache
englisch
Veröffentlichungsjahr
2017
HGF-Berichtsjahr
2017
ISSN (print) / ISBN
0178-2762
e-ISSN
1432-0789
Zeitschrift
Biology and Fertility of Soils
Quellenangaben
Band: 53,
Heft: 5,
Seiten: 485–489
Verlag
Springer
Verlagsort
New York
Begutachtungsstatus
Peer reviewed
POF Topic(s)
30202 - Environmental Health
Forschungsfeld(er)
Environmental Sciences
PSP-Element(e)
G-504700-001
WOS ID
WOS:000403352800002
Scopus ID
85018791819
Erfassungsdatum
2017-09-19