PuSH - Publikationsserver des Helmholtz Zentrums München: Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography.

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Berninger, M.T.* ; Mohajerani, P. ; Wildgruber, M.* ; Bézière, N. ; Kimm, M.A.* ; Ma, X. ; Haller, B.* ; Fleming, M.J.* ; Vogt, S.* ; Anton, M.* ; Imhoff, A.B.* ; Ntziachristos, V. ; Meier, R.* ; Henning, T.D.*

Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography.

Photoacoustics 6, 37-47 (2017)

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The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated. Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT- and Caspase-3/-7-testing in vitro. 2 × 106, 2 × 105 and 2 × 104 MSCs labeled with 5 and 10 μg DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed. Concentrations up to 10 μg DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal. FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model.

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