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TEDDY Study Group (Roth, R. ; Strauss, E.) ; Baxter, J.* ; Vehik, K.* ; Hopkins, D.* ; Killian, M.* ; Gesualdo, P.* ; Melin, J.* ; Simell, B.* ; Lernmark, A.* ; Johnson, S.B.*

The feasibility of salivary sample collection in an international pediatric cohort: The the TEDDY study.

Dev. Psychobiol. 59, 658-667 (2017)

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Saliva offers a relatively noninvasive method for measuring analytes such as cortisol, holding particular promise for use in pediatric populations on a large scale if a rigorous collection protocol is feasible in diverse settings. The Environmental Determinants of Diabetes in the Young study protocol, conducted in centers in the United States, Sweden, Finland, and Germany, used salivary collection to assess cortisol level as a physiologic marker of stress. Saliva was collected using Sorbettes from subjects at 3.5, 4.5, and 5.5 years of age. Parents collected a morning sample, and staff collected pre- and post-blood draw samples. Feasibility was assessed based on protocol completion, adherence with instructions, factors affecting adherence, and sufficiency of saliva sample for cortisol determination. Collection of saliva samples in a diverse pediatric population is feasible. Establishing non-invasive and acceptable methods for collecting physiological parameters of stress will allow better exploration of determinants of health in this important population.

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