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Loebel, M.* ; Eckey, M.* ; Sotzny, F.* ; Hahn, E.G.* ; Bauer, S.* ; Grabowski, P.* ; Zerweck, J.* ; Holenya, P.* ; Hanitsch, L.G.* ; Wittke, K.* ; Borchmann, P.* ; Rüffer, J.U.* ; Hiepe, F.* ; Ruprecht, K.* ; Behrends, U. ; Meindl, C. ; Volk, H.D.* ; Reimer, U.* ; Scheibenbogen, C.*

Serological profiling of the EBV immune response in Chronic Fatigue Syndrome using a peptide microarray.

PLoS ONE 12:e0179124 (2017)
Verlagsversion Forschungsdaten DOI
Open Access Gold
Creative Commons Lizenzvertrag
Background: Epstein-Barr-Virus (EBV) plays an important role as trigger or cofactor for various autoimmune diseases. In a subset of patients with Chronic Fatigue Syndrome (CFS) disease starts with infectious mononucleosis as late primary EBV-infection, whereby altered levels of EBVspecific antibodies can be observed in another subset of patients. Methods: We performed a comprehensive mapping of the IgG response against EBV comparing 50 healthy controls with 92 CFS patients using a microarray platform. Patients with multiple sclerosis (MS), systemic lupus erythematosus (SLE) and cancer-related fatigue served as controls. 3054 overlapping peptides were synthesised as 15-mers from 14 different EBV proteins. Array data was validated by ELISA for selected peptides. Prevalence of EBV serotypes was determined by qPCR from throat washing samples. Results: EBV type 1 infections were found in patients and controls. EBV seroarray profiles between healthy controls and CFS were less divergent than that observed for MS or SLE. We found significantly enhanced IgG responses to several EBNA-6 peptides containing a repeat sequence in CFS patients compared to controls. EBNA-6 peptide IgG responses correlated well with EBNA-6 protein responses. The EBNA-6 repeat region showed sequence homologies to various human proteins. Conclusion: Patients with CFS had a quite similar EBV IgG antibody response pattern as healthy controls. Enhanced IgG reactivity against an EBNA-6 repeat sequence and against EBNA-6 protein is found in CFS patients. Homologous sequences of various human proteins with this EBNA-6 repeat sequence might be potential targets for antigenic mimicry.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Epstein-barr-virus; Systemic-lupus-erythematosus; Cells In-vivo; Epithelial-cells; Antibody-responses; Lymphoproliferative Disorders; Nasopharyngeal Carcinoma; Rheumatoid-arthritis; Multiple-sclerosis; Peripheral-blood
Sprache englisch
Veröffentlichungsjahr 2017
HGF-Berichtsjahr 2017
ISSN (print) / ISBN 1932-6203
Zeitschrift PLoS ONE
Quellenangaben Band: 12, Heft: 6, Seiten: , Artikelnummer: e0179124 Supplement: ,
Verlag Public Library of Science (PLoS)
Verlagsort Lawrence, Kan.
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Gene Vector (AGV)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) G-501500-001
DOI 10.1371/journal.pone.0179124
WOS ID WOS:000403088400028
Scopus ID 85020702009
Erfassungsdatum 2017-07-26
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