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Wefers, B. ; Bashir, S.* ; Rossius, J.* ; Wurst, W. ; Kühn, R.*

Gene editing in mouse zygotes using the CRISPR/Cas9 system.

Methods 121-122, 55-67 (2017)
Postprint DOI PMC
Open Access Green
The generation of targeted mouse mutants is a key technology for biomedical research. Using the CRISPR/Cas9 system for induction of targeted double-strand breaks, gene editing can be performed in a single step directly in mouse zygotes. This article covers the design of knockout and knockin alleles, preparation of reagents, microinjection or electroporation of zygotes and the genotyping of pups derived from gene editing projects. In addition we include a section for the control of experimental settings by targeting the Rosa26 locus and PCR based genotyping of blastocysts.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Cas9 ; Crispr ; Gene Editing ; Mouse ; Zygotes; Off-target Sites; Human-cells; Crispr-cas9 Nucleases; Cas9 Nucleases; Mutant Mice; Guide Rnas; Genome; Generation; Specificity; Tool
ISSN (print) / ISBN 1046-2023
e-ISSN 1095-9130
Zeitschrift Methods
Quellenangaben Band: 121-122, Heft: , Seiten: 55-67 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Amsterdam [u.a.]
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Developmental Genetics (IDG)