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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Preparation of decellularized lung matrices for cell culture and protein analysis.
Methods Mol. Biol. 1627, 253-283 (2017)
The limited available treatment options for patients with chronic lung diseases, such as fibrosis, lead to poor prognosis after diagnosis and short survival rates. An exciting new bioengineering approach utilizes de- and recellularization of lung tissue to potentially overcome donor organ shortage and immune reactions toward the received transplant. The goal of decellularization is to create a scaffold which contains the necessary framework for stability and functionality for regenerating lung tissue while removing immunomodulatory factors by removal of cells. After decellularization, the scaffold could be re-functionalized by repopulation with the patient's own stem/progenitor cells to create a fully functional organ or can be used as ex vivo models of disease. In this chapter the decellularization of lung tissue from multiple species (i.e., rodents, pigs, and humans) as well as disease states such as fibrosis is described. We discuss and describe the various quality control measures which should be used to characterize decellularized scaffolds, methods for protein analysis of the remaining scaffold, and methods for recellularization of scaffolds.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Biomaterial ; Decellularization ; Lung ; Mass Spectrometry ; Proteomics ; Scaffold ; Tissue Engineering
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Konferenztitel
Fibrosis
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 1627,
Seiten: 253-283
Verlag
Springer
Verlagsort
Berlin [u.a.]
Begutachtungsstatus
Peer reviewed
Institut(e)
Research Unit Lung Repair and Regeneration (LRR)