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Fereidouni, F.* ; Gorpas, D. ; Ma, D.* ; Fatakdawala, H.* ; Marcu, L.*

Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: A comparative study.

Methods Appl. Fluoresc. 5:035003 (2017)
Postprint DOI PMC
Open Access Green
Fluorescence lifetime imaging has been shown to serve as a valuable tool for interrogating and diagnosis of biological tissue at a mesoscopic level. The ability to analyze fluorescence decay curves to extract lifetime values in real-time is crucial for clinical translation and applications such as tumor margin delineation or intracoronary imaging of atherosclerotic plaques. In this work, we compare the performance of two popular non-parametric (fit-free) methods for determining lifetime values from fluorescence decays in real-time-the Phasor approach and Laguerre deconvolution. We demonstrate results from simulated and experimental data to compare the accuracy and speed of both methods and their dependence on noise and model parameters.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Fluorescence ; Lifetime Imaging ; Laguerre Deconvolution ; Phasor Analysis; Imaging Microscopy; Decay Curves; Frequency-domain; Spectral Images; Living Cells; Time-domain; Vivo; Expansion; Representation; Spectroscopy
ISSN (print) / ISBN 2050-6120
e-ISSN 2050-6120
Zeitschrift Methods and applications in fluorescence
Quellenangaben Band: 5, Heft: 3, Seiten: , Artikelnummer: 035003 Supplement: ,
Verlag Institute of Physics Publishing (IOP)
Verlagsort Bristol
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)