PuSH - Publikationsserver des Helmholtz Zentrums München: Mass spectrometry-based phytochemical screening for hypoglycemic activity of Fagioli di Sarconi beans (<em>Phaseolus vulgaris L.</em>).

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Pascale, R.* ; Cataldi, T.R.I.* ; Bianco, G.* ; Schmitt-Kopplin, P. ; Bosco, F.* ; Vignola, L.* ; Uhl, J. ; Lucio, M. ; Milella, L.*

Mass spectrometry-based phytochemical screening for hypoglycemic activity of Fagioli di Sarconi beans (Phaseolus vulgaris L.).

Food Chem. 242, 497-504 (2017)

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The present study deals with the evaluation of antidiabetic activities of Fagioli di Sarconi beans (Phaseolus vulgaris), including 21 ecotypes protected by the European Union with the mark PGI (i.e., Protected Geographical Indication), and cultivated in Basilicata (southern Italy). For this purpose, α-glucosidase and α-amylase assays were assessed; among all bean ecotypes, the tight green seed colour of Verdolino extracts exhibited the highest α-glucosidase and α-amylase inhibitory activity with IC₅₀ = 1.1 ± 0.1 μg/ml and IC₅₀ = 19.3 ± 1.1 μg/ml, respectively. Phytochemical compound screening of all Fagioli di Sarconi beans performed by flow injection-electrospray ionization-ultrahigh resolution mass spectrometry (uHRMS) and based on the calculation of elemental formulas from accurate m/z values, was helpful to annotate specific compounds, such as alkaloids, saponins, flavonoids, and terpenoids, which are most likely responsible for their biological activity.

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