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Roberts, S. ; Seeger, M. ; Jiang, Y. ; Mishra, A. ; Sigmund, F. ; Stelzl, A. ; Lauri, A. ; Symvoulidis, P. ; Rolbieski, H.* ; Preller, M.* ; Dean-Ben, X.L. ; Razansky, D. ; Orschmann, T.* ; Desbordes, S.C. ; Vetschera, P.* ; Bach, T.* ; Ntziachristos, V. ; Westmeyer, G.G.

Calcium sensor for photoacoustic imaging.

J. Am. Chem. Soc. 140, 2718-2721 (2018)
Postprint DOI PMC
Open Access Green
We introduce a selective and cell-permeable calcium sensor for photoacoustics (CaSPA), a versatile imaging technique that allows for fast volumetric mapping of photoabsorbing molecules with deep tissue penetration. To optimize for Ca2+-dependent photoacoustic signal changes, we synthesized a selective metallochromic sensor with high extinction coefficient, low quantum yield, and high photobleaching resistance. Micromolar concentrations of Ca2+ lead to a robust blueshift of the absorbance of CaSPA, which translated into an accompanying decrease of the peak photoacoustic signal. The acetoxymethyl esterified sensor variant was readily taken up by cells without toxic effects and thus allowed us for the first time to perform live imaging of Ca2+ fluxes in genetically unmodified cells and heart organoids as well as in zebrafish larval brain via combined fluorescence and photoacoustic imaging.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Arsenazo-iii; Indicators; Microscopy; Ca-2
ISSN (print) / ISBN 0002-7863
e-ISSN 1520-5126
Zeitschrift Journal of the American Chemical Society
Quellenangaben Band: 140, Heft: 8, Seiten: 2718-2721 Artikelnummer: , Supplement: ,
Verlag American Chemical Society (ACS)
Verlagsort Washington
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)
Institute of Developmental Genetics (IDG)