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Desbenoit, N.* ; Walch, A.K. ; Spengler, B.* ; Brunelle, A.* ; Römpp, A.*

Correlative mass spectrometry imaging, applying TOF-SIMS and AP-MALDI to a single tissue section.

Rapid Commun. Mass Spectrom. 32, 159-166 (2017)
Postprint DOI PMC
Open Access Green
RATIONALE: Mass spectrometry imaging (MSI) is a powerful tool for mapping the surface of a sample. Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) and Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization (AP-MALDI) offer complementary capabilities. Here, we present a workflow to apply both techniques to a single tissue section and combine the resulting data on the example of human colon cancer tissue. METHODS: Following cryo-sectioning, images were acquired using the high spatial resolution (1 μm pixel size) provided by TOF-SIMS. The same section was then coated with a para-nitroaniline matrix and images were acquired using AP-MALDI coupled to an Orbitrap mass spectrometer, offering high mass resolution, high mass accuracy and MS/MS capabilities. Datasets provided by both mass spectrometers were converted into the open and vendor-independent imzML file format and processed with the open-source software MSiReader. RESULTS: The TOF-SIMS and AP-MALDI-MS mass spectra show strong signals of fatty acids, cholesterol, phosphatidylcholine and sphingomyelin. We showed a high correlation between the fatty acid ions detected with TOF-SIMS in negative ion mode and the phosphatidylcholine ions detected with AP-MALDI in positive ion mode using a similar setting for visualization. Histological staining on the same section allowed the identification of the anatomical structures and their correlation with the ion images. CONCLUSIONS: This multimodal approach using two MSI platforms shows an excellent complementarity for the localization and identification of lipids. The spatial resolution of both systems is at or close to cellular dimensions and thus spatial correlation can only be obtained if the same tissue section is analyzed sequentitially. imzML-based data processing allows a real correlation of the imaging datasets provided by these two technologies and opens the way for a more complete molecular view of the anatomical structures of biological tissues.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Cluster-tof-sims; High-resolution; Biological Tissue; Maldi-tof; Surface-analysis; Brain-tissue; Ms; Phospholipids; Distributions; Bombardment
ISSN (print) / ISBN 0951-4198
e-ISSN 1097-0231
Zeitschrift Rapid Communications in Mass Spectrometry
Quellenangaben Band: 32, Heft: 2, Seiten: 159-166 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Hoboken
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Analytical Pathology (AAP)