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Bryce, M. ; Drews, O.* ; Schenk, M.F.* ; Menzel, A.* ; Estrella, N.* ; Weichenmeier, I. ; Smulders, M.J.* ; Buters, J.T.M. ; Ring, J. ; Görg, A.* ; Behrendt, H. ; Traidl-Hoffmann, C.

Impact of urbanization on the proteome of birch pollen and its chemotactic activity on human granulocytes.

Int. Arch. Allergy Immunol. 151, 46-55 (2010)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Epidemiologic studies reveal a dramatic increase in allergies in the last decades. Air pollution is considered to be one of the factors responsible for this augmentation. The aim of this study was to analyze the impact of urbanization on birch pollen. The birch pollen proteome was investigated in order to identify differences in protein abundance between pollen from rural and urban areas. The allergenicity of birch pollen from both areas was evaluated by assessing its chemotactic potency as well as its protein and allergen contents.Difference gel electrophoresis (DIGE) was used to analyze the pollen proteome. The chemotactic activity of aqueous pollen extracts was determined by migration assays of human neutrophils.RESULTS: DIGE revealed 26 differences in protein spot intensity between pollen from urban and rural areas. One of these proteins was identified by de novo sequencing as the 14-3-3 protein, which resembles a stress-induced factor in other plant species. Furthermore, extracts from pollen collected in urban areas had higher chemotactic activity on human neutrophils compared to pollen from rural sites.CONCLUSIONS: The present study points to an impact of air pollution on allergen carrier proteome and release of chemotactic substances. The increment in proinflammatory substances such as pollen-associated lipid mediators might contribute to the described urban-rural gradient of allergy prevalence. Furthermore, our study suggests that allergenicity is determined by more than the sole allergen content.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Allergy; Birch pollen; Difference gel electrophoresis; Pollution;Two-dimensional polyacrylamide electrophoresis
ISSN (print) / ISBN 1018-2438
e-ISSN 1423-0097
Zeitschrift International Archives of Allergy and Immunology
Quellenangaben Band: 151, Heft: 1, Seiten: 46-55 Artikelnummer: , Supplement: ,
Verlag Karger
Verlagsort Basel ; Freiburg [Breisgau] ; Paris ; London ; New York ; Bangalore ; Bangkok ; Singapore ; Tokyo ; Sydney
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) CCG Environmental Dermatology and Allergology (ILBD-KAU)
Institute of Epidemiology (EPI)