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Karakatsani, A.* ; Marichal, N.* ; Urban, S.* ; Kalamakis, G.* ; Ghanem, A.* ; Schick, A.J.* ; Zhang, Y.* ; Conzelmann, K.* ; Rüegg, M.A.* ; Berninger, B.* ; de Almodovar, C.R.* ; Gascón, S. ; Kroeger, S.*

Neuronal LRP4 regulates synapse formation in the developing CNS.

Development 144, 4604-4615 (2017)
Verlagsversion Forschungsdaten DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The low-density lipoprotein receptor-related protein 4 (LRP4) is essential in muscle fibers for the establishment of the neuromuscular junction. Here, we show that LRP4 is also expressed by embryonic cortical and hippocampal neurons, and that downregulation of LRP4 in these neurons causes a reduction in density of synapses and number of primary dendrites. Accordingly, overexpression of LRP4 in cultured neurons had the opposite effect inducing more but shorter primary dendrites with an increased number of spines. Transsynaptic tracing mediated by rabies virus revealed a reduced number of neurons presynaptic to the cortical neurons in which LRP4 was knocked down. Moreover, neuron-specific knockdown of LRP4 by in utero electroporation of LRP4 miRNA in vivo also resulted in neurons with fewer primary dendrites and a lower density of spines in the developing cortex and hippocampus. Collectively, our results demonstrate an essential and novel role of neuronal LRP4 in dendritic development and synaptogenesis in the CNS.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Lrp4 ; Central Nervous System Development ; Synapse Formation ; Dendritogenesis ; Transsynaptic Tracing ; Agrin ; In Utero Electroporation ; Psd95 ; Bassoon ; Mouse; Neuromuscular-junction Formation; Receptor-related Protein-4; Hippocampal-neurons; Kinase-ii; Agrin; Expression; Muscle; Cortex; Mechanisms; Plasticity
ISSN (print) / ISBN 0950-1991
e-ISSN 1477-9129
Zeitschrift Development / Company of Biologists
Quellenangaben Band: 144, Heft: 24, Seiten: 4604-4615 Artikelnummer: , Supplement: ,
Verlag Company of Biologists
Verlagsort Cambridge
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Stem Cell Research (ISF)