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Chrétien, D.* ; Bénit, P.* ; Ha, H.H.* ; Keipert, S. ; El-Khoury, R.* ; Chang, Y.T.* ; Jastroch, M. ; Jacobs, H.T.* ; Rustin, P.* ; Rak, M.*

Mitochondria are physiologically maintained at close to 50 °C.

PLoS Biol. 16:e2003992 (2018)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
In endothermic species, heat released as a product of metabolism ensures stable internal temperature throughout the organism, despite varying environmental conditions. Mitochondria are major actors in this thermogenic process. Part of the energy released by the oxidation of respiratory substrates drives ATP synthesis and metabolite transport, but a substantial proportion is released as heat. Using a temperature-sensitive fluorescent probe targeted to mitochondria, we measured mitochondrial temperature in situ under different physiological conditions. At a constant external temperature of 38 °C, mitochondria were more than 10 °C warmer when the respiratory chain (RC) was fully functional, both in human embryonic kidney (HEK) 293 cells and primary skin fibroblasts. This differential was abolished in cells depleted of mitochondrial DNA or treated with respiratory inhibitors but preserved or enhanced by expressing thermogenic enzymes, such as the alternative oxidase or the uncoupling protein 1. The activity of various RC enzymes was maximal at or slightly above 50 °C. In view of their potential consequences, these observations need to be further validated and explored by independent methods. Our study prompts a critical re-examination of the literature on mitochondria.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Subcellular Thermal-changes; Fluorescent Protein Mutant; Single-cell Thermometry; Isocitrate Dehydrogenase; Complex-i; Gap Issue; Thermosensors; Respiration; Expression
Sprache englisch
Veröffentlichungsjahr 2018
HGF-Berichtsjahr 2018
ISSN (print) / ISBN 1544-9173
e-ISSN 1545-7885
Zeitschrift PLoS Biology
Quellenangaben Band: 16, Heft: 1, Seiten: , Artikelnummer: e2003992 Supplement: ,
Verlag Public Library of Science (PLoS)
Verlagsort San Francisco
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Obesity (IDO)
POF Topic(s) 90000 - German Center for Diabetes Research
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-501900-221
DOI 10.1371/journal.pbio.2003992
WOS ID WOS:000423830300015
Scopus ID 85041744973
PubMed ID 29370167
Erfassungsdatum 2018-03-19
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