PuSH - Publikationsserver des Helmholtz Zentrums München: Dosimetric quantification of the incidental irradiation of the "true' (deep) ano-inguinal lymphatic drainage of anal cancer patients not described in conventional contouring guidelines.

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Dapper, H.* ; Habl, G.* ; Hirche, C.* ; Münch, S.* ; Oechsner, M.* ; Mayinger, M.* ; Sauter, C.* ; Combs, S.E. ; Habermehl, D.

Dosimetric quantification of the incidental irradiation of the "true' (deep) ano-inguinal lymphatic drainage of anal cancer patients not described in conventional contouring guidelines.

Acta Oncol. 57, 825-830 (2018)

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Flatworms of the species Schmidtea mediterranea are immortal-adult animals contain a large pool of pluripotent stem cells that continuously differentiate into all adult cell types. Therefore, single-cell transcriptome profiling of adult animals should reveal mature and progenitor cells. By combining perturbation experiments, gene expression analysis, a computational method that predicts future cell states from transcriptional changes, and a lineage reconstruction method, we placed all major cell types onto a single lineage tree that connects all cells to a single stem cell compartment. We characterized gene expression changes during differentiation and discovered cell types important for regeneration. Our results demonstrate the importance of single-cell transcriptome analysis for mapping and reconstructing fundamental processes of developmental and regenerative biology at high resolution.

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3.473

1.063