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Pekkanen, J.* ; Valkonen, M.* ; Täubel, M.* ; Tischer, C.* ; Leppanen, H.* ; Karkkainen, P.M.* ; Rintala, H.* ; Zock, J.P.* ; Casas, L.* ; Probst-Hensch, N.* ; Forsberg, B.* ; Holm, M.* ; Janson, C.* ; Pin, I.* ; Gislason, T.* ; Jarvis, D.* ; Heinrich, J. ; Hyvärinen, A.*

Indoor bacteria and asthma in adults: A multicentre case-control study within ECRHS II.

Eur. Respir. J. 51:1701241 (2018)
Verlagsversion DOI PMC
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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Both protective and adverse effects of indoor microbial exposure on asthma have been reported, but mostly in children. To date, no study in adults has used non-targeted methods for detection of indoor bacteria followed by quantitative confirmation. A cross-sectional study of 198 asthmatic and 199 controls was conducted within the European Community Respiratory Health Survey (ECRHS) II. DNA was extracted from mattress dust for bacterial analysis using denaturing gradient gel electrophoresis (DGGE). Selected bands were sequenced and associations with asthma confirmed with four quantitative PCR (qPCR) assays. 15 out of 37 bands detected with DGGE, which had at least a suggestive association (p<0.25) with asthma, were sequenced. Of the four targeted qPCRs, Clostridium cluster XI confirmed the protective association with asthma. The association was dose dependent (aOR 0.43 (95% CI 0.22-0.84) for the fourth versus first quartile, p for trend 0.009) and independent of other microbial markers. Few significant associations were observed for the three other qPCRs used. In this large international study, the level of Clostridium cluster XI was independently associated with a lower risk of prevalent asthma. Results suggest the importance of environmental bacteria also in adult asthma, but need to be confirmed in future studies.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Respiratory-health-survey; Real-time Pcr; In-house Dust; Childhood Asthma; Ribosomal-rna; Microbial Exposure; Birth Cohort; Early-life; Children; Population
ISSN (print) / ISBN 0903-1936
e-ISSN 1399-3003
Zeitschrift European Respiratory Journal
Quellenangaben Band: 51, Heft: 2, Seiten: , Artikelnummer: 1701241 Supplement: ,
Verlag European Respiratory Society
Verlagsort Sheffield
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Epidemiology (EPI)