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Stocki, P.* ; Morris, N.J.* ; Preisinger, C.* ; Wang, X.N.* ; Kolch, W.* ; Multhoff, G. ; Dickinson, A.M.*

Identification of potential HLA class I and class II epitope precursors associated with heat shock protein 70 (HSPA).

Cell Stress Chaperones 15, 729-741 (2010)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Heat shock protein 70 (HSPA) is a molecular chaperone which has been suggested to shuttle human leukocyte antigen (HLA) epitope precursors from the proteasome to the transporter associated with antigen processing. Despite the reported observations that peptides chaperoned by HSPA are an effective source of antigens for cross-priming, little is known about the peptides involved in the process. In this study, we investigated the possible involvement of HSPA in HLA class I or class II antigen presentation and analysed the antigenic potential of the associated peptides. HSPA was purified from CCRF-CEM and K562 cell lines, and using mass spectrometry techniques, we identified 44 different peptides which were co-purified with HSPA. The affinity of the identified peptides to two HSPA isoforms, HSPA1A and HSPA8, was confirmed using a peptide array. Four of the HSPA-associated peptides were matched with 13 previously reported HLA epitopes. Of these 13 peptides, nine were HLA class I and four were HLA class II epitopes. These results demonstrate the association of HSPA with HLA class I and class II epitopes, therefore providing further evidence for the involvement of HSPA in the antigen presentation process.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Heat shock protein 70 (HSPA); Human leukocyte antigen (HLA); Antigen presentation; Molecular chaperone
ISSN (print) / ISBN 1355-8145
e-ISSN 1466-1268
Zeitschrift Cell Stress and Chaperones
Quellenangaben Band: 15, Heft: 5, Seiten: 729-741 Artikelnummer: , Supplement: ,
Verlag Springer
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) CCG Innate Immunity in Tumor Biology (PATH-KTB)