PuSH - Publikationsserver des Helmholtz Zentrums München

Oshima, M.* ; Knoch, K.-P. ; Diedisheim, M.* ; Petzold, A. ; Cattan, P.* ; Bugliani, M.* ; Marchetti, P.* ; Choudhary, P.* ; Huang, G.C.* ; Bornstein, S.R.* ; Solimena, M. ; Albagli-Curiel, O.* ; Scharfmann, R.*

Virus-like infection induces human β cell dedifferentiation.

JCI insight 3:97732 (2018)
Verlagsversion DOI PMC
Open Access Gold
Type 1 diabetes (T1D) is a chronic disease characterized by an autoimmune-mediated destruction of insulin-producing pancreatic β cells. Environmental factors such as viruses play an important role in the onset of T1D and interact with predisposing genes. Recent data suggest that viral infection of human islets leads to a decrease in insulin production rather than β cell death, suggesting loss of β cell identity. We undertook this study to examine whether viral infection could induce human β cell dedifferentiation. Using the functional human β cell line EndoC-βH1, we demonstrate that polyinosinic-polycytidylic acid (PolyI:C), a synthetic double-stranded RNA that mimics a byproduct of viral replication, induces a decrease in β cell-specific gene expression. In parallel with this loss, the expression of progenitor-like genes such as SOX9 was activated following PolyI:C treatment or enteroviral infection. SOX9 was induced by the NF-κB pathway and also in a paracrine non-cell-autonomous fashion through the secretion of IFN-α. Lastly, we identified SOX9 targets in human β cells as potentially new markers of dedifferentiation in T1D. These findings reveal that inflammatory signaling has clear implications in human β cell dedifferentiation.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Beta Cells ; Diabetes ; Inflammation ; Nf-kappab ; Virology
ISSN (print) / ISBN 2379-3708
e-ISSN 2379-3708
Zeitschrift JCI insight
Quellenangaben Band: 3, Heft: 3, Seiten: , Artikelnummer: 97732 Supplement: ,
Verlag Clarivate
Verlagsort Ann Arbor, Michigan
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pancreatic Islet Research (IPI)