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Short-term overfeeding with dairy cream does not modify gut permeability, the fecal microbiota, or glucose metabolism in young healthy men.
J. Nutr. 148, 77-85 (2018)
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DOI
PMC
Background: High-fat diets (HFDs) have been linked to low-grade inflammation and insulin resistance. Objective: The main purpose of the present study was to assess whether acute overfeeding with an HFD affects insulin sensitivity, gut barrier function, and fecal microbiota in humans. Methods: In a prospective intervention study, 24 healthy men [mean ± SD: age 23.0 ± 2.8 y, body mass index (in kg/m2) 23.0 ± 2.1] received an HFD (48% of energy from fat) with an additional 1000 kcal/d (as whipping cream) above their calculated energy expenditure for 7 d. Insulin sensitivity (hyperinsulinemic euglycemic clamp), gut permeability (sugar and polyethylene glycol absorption tests, plasma zonulin), and gut microbiota profiles (high-throughput 16S rRNA gene sequencing) were assessed before and after overfeeding, and 14 d after intervention. Additionally, inflammation markers such as high-sensitivity C-reactive protein, lipopolysaccharide-binding protein, leptin, high-molecular-weight adiponectin, calprotectin, regulated on activation normal, T cell expressed and secreted (RANTES), and monocyte chemoattractant protein-1 were measured in plasma by ELISA. Finally, lipid parameters were analyzed in serum by a laboratory service. Results: Although participants gained 0.9 ± 0.6 kg (P < 0.001) body weight, overnutrition was not associated with a significant change in insulin sensitivity (M value and glucose disposal). Overfeeding for 7 d resulted in elevated serum total (10.2%), LDL (14.6%) and HDL (14.8%) cholesterol concentrations (P < 0.01). In contrast, fasting plasma triglyceride significantly declined (29.3%) during overfeeding (P < 0.001). In addition, there were no significant changes in inflammatory markers. Urine excretion of 4 sugars and polyethylene glycol, used as a proxy for gut permeability, and plasma concentration of zonulin, a marker of paracellular gut permeability, were unchanged. Moreover, overfeeding was not associated with consistent changes in gut microbiota profiles, but marked alterations were observed in a subgroup of 6 individuals. Conclusions: Our findings suggest that short-term overfeeding with an HFD does not significantly impair insulin sensitivity and gut permeability in normal-weight healthy men, and that changes in dominant communities of fecal bacteria occur only in certain individuals.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Gut Barrier Function ; Gut Permeability ; High-fat Diet ; Hyperinsulinemic Euglycemic Clamp ; Inflammation ; Insulin Sensitivity ; Intestinal Microbiota ; Lipopolysaccharide-binding Protein ; Overfeeding ; Zonulin; High-fat Diet; Increased Intestinal Permeability; Insulin-resistance; Adipose-tissue; Postprandial Endotoxemia; Induced Obesity; Inflammation; Mice; Disease; Intervention
ISSN (print) / ISBN
0022-3166
e-ISSN
1541-6100
Zeitschrift
Journal of Nutrition
Quellenangaben
Band: 148,
Heft: 1,
Seiten: 77-85
Verlag
American Society for Nutrition
Verlagsort
Bethesda
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed