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Treberg, J.R.* ; Munro, D.* ; Jastroch, M.* ; Quijada-Rodriguez, A.R.* ; Kutschke, M. ; Wiens, L.*

Comparing electron leak in vertebrate muscle mitochondria.

Integ. & comp. biol. 58, 495-505 (2018)
Verlagsversion Postprint DOI PMC
Open Access Green
Mitochondrial electron transfer for oxidative ATP regeneration is linked to reactive oxygen species (ROS) production in aerobic eukaryotic cells. Because they can contribute to signaling as well as oxidative damage in cells, these ROS have profound impact for the physiology and survival of the organism. Although mitochondria have been recognized as a potential source for ROS for about 50 years, the mechanistic understanding on molecular sites and processes has advanced recently. Most experimental approaches neglect thermal variability among species although temperature impacts mitochondrial processes significantly. Here we delineate the importance of temperature by comparing muscle mitochondrial ROS formation across species. Measuring the thermal sensitivity of respiration, electron leak rate (ROS formation), and the antioxidant capacity (measured as H2O2 consumption) in intact mitochondria of representative ectothermic and endothermic vertebrate species, our results suggest that using a common assay temperature is inappropriate for comparisons of organisms with differing body temperatures. Moreover, we propose that measuring electron leak relative to the mitochondrial antioxidant capacity (the oxidant ratio) may be superior to normalizing relative to respiration rates or mitochondrial protein for comparisons of mitochondrial metabolism of ROS across species of varying mitochondrial respiratory capacities.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Oxygen Species Production; Hydrogen-peroxide; Skeletal-muscle; Superoxide-production; Brain Mitochondria; H2o2 Metabolism; Complex Ii; Rates; Temperature; Consumption
ISSN (print) / ISBN 1540-7063
e-ISSN 1557-7023
Zeitschrift Integrative and comparative biology
Quellenangaben Band: 58, Heft: 3, Seiten: 495-505 Artikelnummer: , Supplement: ,
Verlag Oxford University Press
Verlagsort Oxford
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Obesity (IDO)