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Völker-Albert, M.C.* ; Schmidt, A.* ; Barth, T.K. ; Forne, I.* ; Imhof, A.*

Detection of histone modification dynamics during the cell cycle by MS-based proteomics.

Methods Mol. Biol. 1832, 61-74 (2018)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
DNA replication and subsequent deposition of nucleosomes is critical for the maintenance of the genome and epigenetic inheritance. Experiments using human tissue culture cells harvested at defined stages of the cell cycle can help to elucidate the mechanism of histone deposition and chromatin assembly in detail. Here, we describe a pulsed-SILAC approach to distinguish newly synthesized and deposited histones during S-phase of the cell cycle from parental “old” histones incorporated in previous replications and to decipher posttranslational histone modifications (PTMs).
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Histone Quantitation ; Mass Spectrometry ; Ptm-analysis ; Silac Labeling
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Zeitschrift Methods in Molecular Biology
Quellenangaben Band: 1832, Heft: , Seiten: 61-74 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Berlin [u.a.]
Begutachtungsstatus Peer reviewed
Institut(e) CF Metabolomics & Proteomics (CF-MPC)