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Wunderlich, R. ; Rühle, P.F.* ; Deloch, L.* ; Rödel, F.* ; Fietkau, R.* ; Gaipl, U.S.* ; Frey, B.*

Ionizing radiation reduces the capacity of activated macrophages to induce T-cell proliferation, but does not trigger dendritic cell-mediated non-targeted effects.

Int. J. Radiat. Biol. 95, 33-43 (2019)
Postprint DOI PMC
Open Access Green
Purpose: Previous investigations revealed influences of irradiation up to 2Gy on the cytokine secretion profile of inflammatory and peritoneal mouse macrophages (pMCYRILLIC CAPITAL LETTER EF). This raised the question if those alterations impact on dendritic cells and consecutive T-cell responses. Further, the impact of irradiation directly on pMCYRILLIC CAPITAL LETTER EF capacity to induce T-cell responses was analyzed. Materials and methods: pMCYRILLIC CAPITAL LETTER EF were LPS-activated, irradiated and the expression of activation markers was assessed. Treated pMCYRILLIC CAPITAL LETTER EF were co-incubated with T-cells to investigate proliferation. To verify modulating properties of pMCYRILLIC CAPITAL LETTER EF supernatants isolated 24 h after irradiation, bone marrow-derived dendritic cells (BMDC) were co-incubated with supernatants and activation markers as well as the BMDC-induced proliferation of T-cells were measured. Results: pMCYRILLIC CAPITAL LETTER EF showed a highly significantly decreased major histocompatibility complexII (MHCII) expression within a dose range from 0.7-2Gy. Further, the proliferation rate of cluster of differentiation 4(+) (CD4(+)) T-cells was decreased after co-incubation particularly with 2 Gy irradiated pMCYRILLIC CAPITAL LETTER EF. The co-incubation of BMDC with supernatants of activated, irradiated pMCYRILLIC CAPITAL LETTER EF significantly reduced the CD40 expression, but did not impact on the BMDC-derived induction of T-cell proliferation. Conclusions: Inflammatory macrophages being exposed to irradiation have the potential to modulate consecutive adaptive immune reactions. But supernatants of irradiated macrophages do not influence the dendritic cells (DC)-mediated induction of T cell proliferation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Low-dose Radiation ; Activated Macrophages ; Dendritic Cells ; Non-targeted Effects ; Inflammation; Tumor-associated Macrophages; Blood Mononuclear-cells; Kappa-b; Synovial Tissue; Inflammatory Macrophages; Colorectal-cancer; Alpha-particles; X-irradiation; In-vitro; Radiotherapy
ISSN (print) / ISBN 0955-3002
e-ISSN 1362-3095
Zeitschrift International Journal of Radiation Biology
Quellenangaben Band: 95, Heft: 1, Seiten: 33-43 Artikelnummer: , Supplement: ,
Verlag Informa Healthcare
Verlagsort 2-4 Park Square, Milton Park, Abingdon Or14 4rn, Oxon, England
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Translational Metabolic Oncology (IDC-TMO)