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Kreyling, W.G. ; Möller, W. ; Holzwarth, U.* ; Hirn, S. ; Wenk, A. ; Schleh, C. ; Schäffler, M. ; Haberl, N. ; Gibson, N.* ; Schittny, J.C.*

Age-dependent rat lung deposition patterns of inhaled 20 manometer gold nanoparticles and their quantitative biokinetics in adult rats.

ACS Nano 12, 7771-7790 (2018)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
The increasing use of gold nanoparticles leads to a possible increase of exposure by inhalation. Therefore, we have studied the deposition patterns of inhaled 20 nm gold nanoparticles (AuNP) in 7-90 day old rats and their biokinetics in 60 day old ones. Wistar-Kyoto rats inhaled intratracheally 20 nm Au-195-radiolabeled AuNP by negative pressure ventilation over 2 h. Immediately afterward lungs were excised, inflated and microwave dried. AuNP deposition was analyzed by single-photon emission computed tomography, computed-tomography and autoradiography. Completely balanced, quantitative biodistributions in major organs and all body tissues and total excretion were analyzed from 1 h to 28 d after inhalation. Intratracheal inhalation caused AuNP deposition predominately in the caudal lungs, independent of age. About 30% AuNP were deposited on airway epithelia and rapidly cleared by mucociliary clearance. About 80% of AuNP deposited in alveoli was relocated from the epithelium into the interstitium within 24 h and was inaccessible to broncho-alveolar lavage. During interstitial long-term retention, re-entrainment within macrophages back onto the lung epithelium and to the larynx and gastrointestinal tract (GIT) dominated AuNP clearance (rate 0.03 d(-1)) In contrast, AuNP-translocation across the air-blood barrier was much smaller leading to persistent retention in secondary organs and tissues in the ranking order liver > soft issue > spleen > kidneys > skeleton > blood > uterus > heart > brain. The age independent, inhomogeneous AuNP deposition was probably caused by the negative pressure ventilation. Long-term AuNP clearance was dominated by macrophage-mediated transport from the interstitium to the larynx and GIT. Translocation across the rat air blood barrier appeared to be similar to that of humans for similar sized AuNP.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Gold Nanoparticle Aerosols ; Intratracheal Inhalation ; Air-blood Barrier Translocation ; Extrapulmonary Nanoparticle Accumulation ; Nanoparticle Clearance ; Alveolar Macrophage ; Interstitial Nanoparticle Relocation ; Epithelial Re-entrainment; Titanium-dioxide Nanoparticles; Pulmonary Macrophagic System; Insoluble Iridium Particles; Long-term Clearance; Ultrafine Particles; In-vivo; Alveolar Macrophages; Acinar Structures; Secondary Organs; Renal Clearance
ISSN (print) / ISBN 1936-0851
e-ISSN 1936-086X
Zeitschrift ACS Nano
Quellenangaben Band: 12, Heft: 8, Seiten: 7771-7790 Artikelnummer: , Supplement: ,
Verlag American Chemical Society (ACS)
Verlagsort 1155 16th St, Nw, Washington, Dc 20036 Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Epidemiology II (EPI2)
Lung Health and Immunity (LHI)