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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Determination of enrichment factors for modified RNA in MeRIP experiments.
Methods 156, 102-109 (2019)
In the growing field of RNA modification, precipitation techniques using antibodies play an important role. However, little is known about their specificities and protocols are missing to assess their effectiveness. Here we present a method to assess enrichment factors after MeRIP-type pulldown experiments, here exemplified with a commercial antibody against N6-methyladenosine (m(6)A). Testing different pulldown and elution conditions, we measure enrichment factors of 4-5 using m(6)A-containing mRNAs against an unmodified control of identical sequence. Both types of mRNA carry P-32 labels at different nucleotides, allowing their relative quantification in a mixture after digestion to nucleotides, separation by TLC and quantitative phosphorimaging of the labels.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Times Cited
Scopus
Cited By
Cited By
Altmetric
3.782
1.039
12
11
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Merip ; Antibody ; Rna Modification ; M(6)a/n6-methyladenosine ; Tlc; Messenger-rna; Methylation; N-6-methyladenosine; Reveals; Translation; M6a
Sprache
Veröffentlichungsjahr
2019
Prepublished im Jahr
2018
HGF-Berichtsjahr
2018
ISSN (print) / ISBN
1046-2023
e-ISSN
1095-9130
Zeitschrift
Methods
Quellenangaben
Band: 156,
Seiten: 102-109
Verlag
Elsevier
Verlagsort
Amsterdam [u.a.]
Begutachtungsstatus
Peer reviewed
Institut(e)
CF Monoclonal Antibodies (CF-MAB)
POF Topic(s)
30201 - Metabolic Health
Forschungsfeld(er)
Helmholtz Diabetes Center
PSP-Element(e)
G-502210-001
WOS ID
WOS:000462417800014
Scopus ID
85056191525
PubMed ID
30394295
Erfassungsdatum
2018-11-07