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Gao, C.* ; Ding, Z.* ; Qian, J.* ; Liu, X.* ; Zhang, X.* ; Cong, Y.* ; Ding, C.* ; Yu, S.* ; Hu, S.* ; Stöger, T. ; Yin, R.*

The virulence of NDV NA-1 strain regulated by the 3 ' leader or 5 ' trailer sequences.

Microb. Pathog. 126, 109-115 (2019)
Postprint DOI PMC
Open Access Green
The 3' and 5' terminal regions of Newcastle disease virus (NDV) genome are cis-acting regulatory elements involved in replication, transcription, and packaging of genomic and anti-genomic viral RNA. There are 6 different nucleotides (nts) at the 3' and 34 different nts at the 5' end of genome in the velogenic NA-1 strain and lentogenic LaSota strain, sharing 90.00% and 70.18% identity, respectively. We investigated the roles of 3' and 5' terminus in the NA-1 strain in viral replication, virulence and pathogenicity. Three NA-1 strain-based recombinant viruses (rNA-L, rNA-T, and rNA-LT) were generated using reverse genetics by either replacing the 3' leader or 5' trailer sequence of NA-1 strain or both with the corresponding sequences of the LaSota strain. Viral replication kinetics and pathogenicity of rNA-L and rNA-T were indistinguishable to that of the parental NA-1 strain, demonstrating that individual replacement or 3' or 5' terminal sequences had little influence. However, the synchronal replacement of both 3' and 5' terminal sequences resulted in decreased viral plaque size, reduced virulence and weaker pathogenicity in 2-week-old chickens. Therefore, our results suggest that the 3' and 5' terminal sequences of NDV genome could only influence the viral virulence when worked collaboratively, while separate replacement would not alter its biological characteristics.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Newcastle Disease Virus ; Terminal Sequence ; Virulence; Newcastle-disease-virus; Protein; Regions; China; Size
ISSN (print) / ISBN 0882-4010
e-ISSN 1096-1208
Zeitschrift Microbial pathogenesis
Quellenangaben Band: 126, Heft: , Seiten: 109-115 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Oxford
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Lung Health and Immunity (LHI)