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Schulz, S.* ; Pérez-de-Mora, A.* ; Engel, M. ; Munch, J.-C.* ; Schloter, M.

A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil.

J. Microbiol. Methods 80, 295-298 (2010)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The utilization of quantitative PCR (qPCR) approaches such as MPN-qPCR and real-time-qPCR for in situ assessment of functional genes yields substantial quantitative and qualitative differences. We show this by targeting the alkB gene related to biodegradation of aliphatic alkanes in a set of environmental samples with differing hydrocarbon content.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Quantitative PCR; Real-time PCR; MPN-PCR; Alkane monooxygenase gene alkB
ISSN (print) / ISBN 0167-7012
e-ISSN 1872-8359
Zeitschrift Journal of Microbiological Methods
Quellenangaben Band: 80, Heft: 3, Seiten: 295-298 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Amsterdam
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Soil Ecology (IBOE)