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Obermann, J. ; Wagner, F. ; Kociaj, A. ; Zambusi, A. ; Ninkovic, J. ; Hauck, S.M. ; Chapouton, P.

The surface proteome of adult neural stem cells in zebrafish unveils long-range cell-cell connections and age-related changes in responsiveness to IGF.

Stem Cell Rep. 12, 258-273 (2019)
Verlagsversion Forschungsdaten DOI PMC
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In adult stem cell populations, recruitment into division is parsimonious and most cells maintain a quiescent state. How individual cells decide to enter the cell cycle and how they coordinate their activity remains an essential problem to be resolved. It is thus important to develop methods to elucidate the mechanisms of cell communication and recruitment into the cell cycle. We made use of the advantageous architecture of the adult zebrafish telencephalon to isolate the surface proteins of an intact neural stem cell (NSC) population. We identified the proteome of NSCs in young and old brains. The data revealed a group of proteins involved in filopodia, which we validated by a morphological analysis of single cells, showing apically located cellular extensions. We further identified an age-related decrease in insulin-like growth factor (IGF) receptors. Expressing IGF2b induced divisions in young brains but resulted in incomplete divisions in old brains, stressing the role of cell-intrinsic processes in stem cell behavior.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Gfap ; Aging ; Biotinylation ; Filopodia ; Lamellipodia ; Mass Spectrometry ; Neurogenesis ; Pallium ; Quiescence ; Radial Glia ; Telencephalon; Radial Glia; Hypothalamic Neurogenesis; Subventricular Zone; Ependymal Cells; Growth; Brain; Quiescence; Decline; Cycle; Proliferation
Sprache englisch
Veröffentlichungsjahr 2019
HGF-Berichtsjahr 2019
ISSN (print) / ISBN 2213-6711
Zeitschrift Stem Cell Reports
Quellenangaben Band: 12, Heft: 2, Seiten: 258-273 Artikelnummer: , Supplement: ,
Verlag Cell Press
Verlagsort Maryland Heights, MO
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Sensory Biology and Organogenesis (SBO)
CF Metabolomics & Proteomics (CF-MPC)
Institute of Stem Cell Research (ISF)
POF Topic(s) 30204 - Cell Programming and Repair
30203 - Molecular Targets and Therapies
Forschungsfeld(er) Stem Cell and Neuroscience
Enabling and Novel Technologies
PSP-Element(e) G-500100-001
G-505700-001
A-630700-001
G-500800-001
DOI 10.1016/j.stemcr.2018.12.005
WOS ID WOS:000458537400008
Scopus ID 85061027550
PubMed ID 30639211
Erfassungsdatum 2019-02-27
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