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Martin, L.* ; Meier, M. ; Lyons, S.M.* ; Sit, R.V.* ; Marzluff, W.F.* ; Quake, S.R.* ; Chang, H.Y.*

Systematic reconstruction of RNA functional motifs with high-throughput microfluidics.

Nat. Methods 9, 1192-1194 (2012)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretation of phylogenetic data.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Letter to the Editor
Korrespondenzautor
ISSN (print) / ISBN 1548-7091
e-ISSN 1548-7105
Zeitschrift Nature Methods
Quellenangaben Band: 9, Heft: 12, Seiten: 1192-1194 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort New York, NY
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Pioneer Campus (HPC)