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Systematic reconstruction of RNA functional motifs with high-throughput microfluidics.
Nat. Methods 9, 1192-1194 (2012)
We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretation of phylogenetic data.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Letter to the Editor
ISSN (print) / ISBN
1548-7091
e-ISSN
1548-7105
Zeitschrift
Nature Methods
Quellenangaben
Band: 9,
Heft: 12,
Seiten: 1192-1194
Verlag
Nature Publishing Group
Verlagsort
New York, NY
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Helmholtz Pioneer Campus (HPC)