OpenSSL SSL_connect: Connection reset by peer in connection to v2.sherpa.ac.uk:443 PuSH - Publikationsserver des Helmholtz Zentrums München: Investigating age-related changes in proliferation and the cell division repertoire of parenchymal reactive astrocytes.

PuSH - Publikationsserver des Helmholtz Zentrums München

Heimann, G.* ; Sirko, S.

Investigating age-related changes in proliferation and the cell division repertoire of parenchymal reactive astrocytes.

In: Astrocytes. Berlin [u.a.]: Springer, 2019. 277-292 (Methods Mol. Biol. ; 1938)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Reactive gliosis is a complicated process involving all types of glial cells and is the therapeutic target of efforts to treat several types of neuropathologies. Parenchymal astrocytes continuously survey their microenvironment to identify even tiny abnormalities in the central nervous system (CNS) homeostasis and react rapidly to brain damage, such as following ischemia, trauma, or neurodegenerative diseases, to prevent propagation of tissue damage. Aging can play causal roles in certain astroglial dysfunctions, however, still little is known to what extent the heterogeneous reaction of astrocytes at the injury site might be impaired over the course of aging. Based on our experience with both in vitro and in vivo experimental paradigms, we describe here in detail the analysis of age-related changes in (1) proliferative response of parenchymal astrocytes within the posttraumatic cerebral cortex grey matter (GM), and (2) repertoire of their cell divisions in adherent cell culture prepared from the injured GM of young and old double transgenic GFAP-mRFP1/(FUCCI)-S/G2/M-mAG-hGeminin mice by single cell time-lapse imaging.
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Publikationstyp Artikel: Sammelbandbeitrag/Buchkapitel
Korrespondenzautor
Schlagwörter Brain Injury ; Cell Cycle ; Cell Division ; Cell Imaging ; Proliferation ; Reactive Astrocyte
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Bandtitel Astrocytes
Zeitschrift Methods in Molecular Biology
Quellenangaben Band: 1938, Heft: , Seiten: 277-292 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Berlin [u.a.]
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Stem Cell Research (ISF)