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Qian, Y.* ; Piatkevich, K.D.* ; Mc Larney, B. ; Abdelfattah, A.S.* ; Mehta, S.* ; Murdock, M.H.* ; Gottschalk, S. ; Molina, R.S.* ; Zhang, W.* ; Chen, Y.* ; Wu, J.* ; Drobizhev, M.* ; Hughes, T.E.* ; Zhang, J.* ; Schreiter, E.R.* ; Shoham, S.* ; Razansky, D. ; Boyden, E.S.* ; Campbell, R.E.*

A genetically encoded near-infrared fluorescent calcium ion indicator.

Nat. Methods 16, 171-174 (2019)
Postprint DOI PMC
Open Access Green
We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca2+ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca2+ imaging in combination with other optogenetic indicators and actuators.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Proteins
ISSN (print) / ISBN 1548-7091
e-ISSN 1548-7105
Zeitschrift Nature Methods
Quellenangaben Band: 16, Heft: 2, Seiten: 171-174 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort New York, NY
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)