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Lauri, A. ; Soliman, D. ; Omar, M. ; Stelzl, A. ; Ntziachristos, V. ; Westmeyer, G.G.

Whole-cell photoacoustic sensor based on pigment relocalization.

ACS sens. 4, 603-612 (2019)
Verlagsversion Forschungsdaten DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
Photoacoustic (optoacoustic) imaging can extract molecular information with deeper tissue penetration than possible by fluorescence microscopy techniques. However, there is currently still a lack of robust genetically controlled contrast agents and molecular sensors that can dynamically detect biological analytes of interest with photoacoustics. In a biomimetic approach, we took inspiration from cuttlefish who can change their color by relocalizing pigment-filled organelles in so-called chromatophore cells under neurohumoral control. Analogously, we tested the use of melanophore cells from Xenopus laevis, containing compartments (melanosomes) filled with strongly absorbing melanin, as whole-cell sensors for optoacoustic imaging. Our results show that pigment relocalization in these cells, which is dependent on binding of a ligand of interest to a specific G protein-coupled receptor (GPCR), can be monitored in vitro and in vivo using photoacoustic mesoscopy. In addition to changes in the photoacoustic signal amplitudes, we could furthermore detect the melanosome aggregation process by a change in the frequency content of the photoacoustic signals. Using bioinspired engineering, we thus introduce a photoacoustic pigment relocalization sensor (PaPiReS) for molecular photoacoustic imaging of GPCR-mediated signaling molecules.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Photoacoustics ; Optoacoustics ; Whole-cell Sensor ; G Protein-coupled Receptor Signaling ; Gpcr Signaling ; Melanophores ; Photoacoustic Frequency Spectrum ; Molecular Imaging; Dispersion; Melanophores; Aggregation; Tomography; Expression; Dopamine; Receptor; Light
Sprache englisch
Veröffentlichungsjahr 2019
HGF-Berichtsjahr 2019
ISSN (print) / ISBN 2379-3694
e-ISSN 2379-3694
Zeitschrift ACS sensors
Quellenangaben Band: 4, Heft: 3, Seiten: 603-612 Artikelnummer: , Supplement: ,
Verlag American Chemical Society (ACS)
Verlagsort Washington, DC
Begutachtungsstatus Peer reviewed
Institut(e) Insitute of Synthetic Biomedicine (ISBM)
Institute of Biological and Medical Imaging (IBMI)
Institute of Developmental Genetics (IDG)
POF Topic(s) 30205 - Bioengineering and Digital Health
30204 - Cell Programming and Repair
Forschungsfeld(er) Enabling and Novel Technologies
Genetics and Epidemiology
PSP-Element(e) G-509300-001
G-505500-001
G-505592-001
G-500500-001
DOI 10.1021/acssensors.8b01319
WOS ID WOS:000462554200010
Scopus ID 85060606542
PubMed ID 30663315
Erfassungsdatum 2019-03-01
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