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Assessing calcium-stimulated mitochondrial bioenergetics using the seahorse XF96 analyzer.

In: Calcium Signalling. Berlin [u.a.]: Springer, 2019. 197-222 (Methods Mol. Biol. ; 1925)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The development of fluorescence-based oxygen sensors coupled with microplate-based assays for quantitative bioenergetics analyses enables screening multiple experimental conditions at once with small biological material and in a timely manner. In this chapter, we outline detailed protocols and practical tips to design and perform controlled measurements of (a) respiratory and glycolytic metabolism of intact cells, (b) substrate-dependent respiration in permeabilized cells and isolated mitochondria, and (c) calcium-dependent regulation of mitochondrial bioenergetics with Seahorse XF Flux Analyzers.
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Publikationstyp Artikel: Sammelbandbeitrag/Buchkapitel
Korrespondenzautor
Schlagwörter Bioenergetics ; Calcium ; Intact Cells ; Mitochondria ; Oxygen Consumption ; Permeabilized Cells ; Respiration ; Seahorse Xf Analyzer
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Bandtitel Calcium Signalling
Quellenangaben Band: 1925, Heft: , Seiten: 197-222 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Berlin [u.a.]
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed