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Lindermayr, C. ; Sell, S. ; Müller, B.* ; Leister, D.* ; Durner, J.

Redox regulation of the NPR1-TGA1 system of Arabidopsis thaliana by nitric oxide.

Plant Cell 22, 2894-2907 (2010)
Verlagsversion Volltext DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The role of reactive oxygen and nitrogen species in local and systemic defense reactions is well documented. NPR1 and TGA1 are key redox-controlled regulators of systemic acquired resistance in plants. NPR1 monomers interact with the reduced form of TGA1, which targets the activation sequence-1 (as-1) element of the promoter region of defense proteins. Here, we report the effect of the physiological nitric oxide donor S-nitrosoglutathione on the NPR1/TGA1 regulation system in Arabidopsis thaliana. Using the biotin switch method, we demonstrate that both NPR1 and TGA1 are S-nitrosylated after treatment with S-nitrosoglutathione. Mass spectrometry analyses revealed that the Cys residues 260 and 266 of TGA1 are S-nitrosylated and S-glutathionylated even at GSNO concentrations in the low micromolar range. Furthermore, we showed that S-nitrosoglutathione protects TGA1 from oxygen-mediated modifications and enhances the DNA binding activity of TGA1 to the as-1 element in the presence of NPR1. In addition, we observed that the translocation of NPR1 into the nucleus is promoted by nitric oxide. Taken together, our results suggest that nitric oxide is a redox regulator of the NPR1/TGA1 system and that they underline the importance of nitric oxide in the plant defense response.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Protein S-nitrosylation; Plant-disease resistance; DNA-binding activity; Transcription factors; Salicylic-acid; Acquired-resistance; Microarray analysis; Nitrosative stress; Cysteine residues; Guanylyl cyclase
ISSN (print) / ISBN 1040-4651
e-ISSN 1532-298X
Zeitschrift The Plant cell
Quellenangaben Band: 22, Heft: 8, Seiten: 2894-2907 Artikelnummer: , Supplement: ,
Verlag American Society of Plant Biologists (ASPB)
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biochemical Plant Pathology (BIOP)