möglich sobald bei der ZB eingereicht worden ist.
Design and generation of gene-targeting vectors.
In: Current Protocols in Mouse Biology. Hoboken, NJ: Wiley-Blackwell, 2011. 199-211 ( ; 1)
This unit provides an overview of the major types of mutant alleles that can be generated by gene targeting in ES cells. It presents the growing public resources of premade gene targeting vectors, modified ES cells, and mutant mice. General guidelines for the design of targeting vectors are followed by protocols for the construction of vectors to generate knockout (KO), conditional KO, and subtle mutant alleles.
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Publikationstyp
Artikel: Sammelbandbeitrag/Buchkapitel
Schlagwörter
gene targeting; conditional KO mice; targeting vector design; knockout mice; EUCOMM/KOMP
ISBN
9780470942390
Bandtitel
Current Protocols in Mouse Biology
Quellenangaben
Band: 1,
Seiten: 199-211
Verlag
Wiley-Blackwell
Verlagsort
Hoboken, NJ
Institut(e)
Institute of Developmental Genetics (IDG)