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Mishra, K. ; Fuenzalida Werner, J.P. ; Ntziachristos, V. ; Stiel, A.-C.

Photocontrollable proteins for optoacoustic imaging.

Anal. Chem. 91, 5470-5477 (2019)
Postprint DOI PMC
Open Access Green
Photocontrollable proteins revolutionized life-science imaging due to their contribution to subdiffraction-resolution optical microscopy. They might have yet another lasting impact on photo- or optoacoustic imaging (OA). OA combines optical contrast with ultrasound detection enabling high-resolution real-time in vivo imaging well-beyond the typical penetration depth of optical methods. While OA already showed numerous applications relying on endogenous contrast from blood hemoglobin or lipids, its application in the life-science was limited by a lack of labels overcoming the strong signal from the aforementioned endogenous absorbers. Here, a number of recent studies showed that photocontrollable proteins provide the means to overcome this barrier eventually enabling OA to image small cell numbers in a complete organism in vivo. In this Feature article, we introduce the key photocontrollable proteins, explain the basic concepts, and highlight achievements that have been already made.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Review
Korrespondenzautor
Schlagwörter Lock-in Detection; Fluorescent Proteins; Photoacoustic Tomography; Natural Photoreceptors; Performance; Chromophore; Microscopy
ISSN (print) / ISBN 0003-2700
e-ISSN 1520-6882
Zeitschrift Analytical Chemistry
Quellenangaben Band: 91, Heft: 9, Seiten: 5470-5477 Artikelnummer: , Supplement: ,
Verlag American Chemical Society (ACS)
Verlagsort 1155 16th St, Nw, Washington, Dc 20036 Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)