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Timme, S.* ; Schmitt, E.* ; Stein, S.* ; Schwarz-Finsterle, J.* ; Wagner, J.* ; Walch, A.K. ; Werner, M.* ; Hausmann, M.* ; Wiech, T.*

Nuclear position and shape deformation of chromosome 8 territories in pancreatic ductal adenocarcinoma.

Anal. Cell. Pathol. 34, 21-33 (2011)
Verlagsversion DOI PMC
Open Access Gold
Cell type specific radial positioning of chromosome territories (CTs) and their sub-domains in the interphase seem to have functional relevance in non-neoplastic human nuclei, while much less is known about nuclear architecture in carcinoma cells and its development during tumor progression. We analyzed the 3D-architecture of the chromosome 8 territory (CT8) in carcinoma and corresponding non-neoplastic ductal pancreatic epithelium. Fluorescence-in-situ-hybridization (FISH) with whole chromosome painting (WCP) probes on sections from formalin-fixed, paraffin wax-embedded tissues from six patients with ductal adenocarcinoma of the pancreas was used. Radial positions and shape parameters of CT8 were analyzed by 3D-microscopy. None of the parameters showed significant inter-individual changes. CT8 was localized in the nuclear periphery in carcinoma cells and normal ductal epithelial cells. Normalized volume and surface of CT8 did not differ significantly. In contrast, the normalized roundness was significantly lower in carcinoma cells, implying an elongation of neoplastic cell nuclei. Unexpectedly, radial positioning of CT8, a dominant parameter of nuclear architecture, did not change significantly when comparing neoplastic with non-neoplastic cells. A significant deformation of CT8, however, accompanies nuclear atypia of carcinoma cells. This decreased roundness of CTs may reflect the genomic and transcriptional alterations in carcinoma.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Pancreatic adenocarcinoma; nuclear architecture; chromosome 8 territory; radial positioning; shape of chromosome 8 territory
Sprache englisch
Veröffentlichungsjahr 2011
HGF-Berichtsjahr 2011
ISSN (print) / ISBN 0921-8912
e-ISSN 1875-8606
Zeitschrift Analytical Cellular Pathology
Quellenangaben Band: 34, Heft: 1-2, Seiten: 21-33 Artikelnummer: , Supplement: ,
Verlag IOS Press
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pathology (PATH)
Research Unit Analytical Pathology (AAP)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30205 - Bioengineering and Digital Health
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-500300-001
G-500390-001
PubMed ID 21483101
DOI 10.3233/ACP-2011-0004
Scopus ID 79960833409
Erfassungsdatum 2011-09-15
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