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In vivo hybrid microscopy of small model organisms.
Proc. SPIE 11077 (2019)
We present the investigation of in vivo small model organisms, which are well established in biological and biomedical research, using a hybrid multiphoton and optoacoustic microscope (HyMPOM). The unique capabilities of HyMPOM for multimodal and potentially label-free signal acquisition, high resolution, as well as deep and fast imaging allow extraction of detailed information across large areas of living tissue on the microscale. Applying HyMPOM to living zebrafish-like fish larvae allowed exploration of the structural composition of the entire brain, including the brain vasculature and the neuronal network. Applying HyMPOM to the ears of living mice enabled accurate imaging of vasculature, connective tissue, keratinocytes, and sebaceous glands. The hybrid microscope proposed here constitutes a novel approach to explore small model organisms in vivo in great detail by revealing the spatial distribution and interplay of various tissue compartments on the microscale.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
ISSN (print) / ISBN
0277-786X
e-ISSN
1996-756X
Zeitschrift
Proceedings of SPIE
Quellenangaben
Band: 11077
Verlag
SPIE
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Biological and Medical Imaging (IBMI)
Insitute of Synthetic Biomedicine (ISBM)
Institute of Developmental Genetics (IDG)
Insitute of Synthetic Biomedicine (ISBM)
Institute of Developmental Genetics (IDG)