Startseite
English
Erweiterte Suche
Durchblättern nach ...
Publikationen im Überblick
Ansprechpartner
Hilfe
DOI
PMC
 |
|
Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
Imaging the bio-distribution of fluorescent probes using multispectral epi-Illumination cryoslicing imaging.
Mol. Imaging Biol. 13, 874-885 (2011)
Purpose: The increasing availability of fluorescent probes for in vivo optical imaging enables the interrogation of complex biological processes in small animals serving as models for human-like tissue function and disease. However, the validation of probe bio-distribution during their development or the study of different disease models, in support of in vivo imaging studies, is not straightforward. Procedures: The imaging system developed consists of a customized multispectral planar imager that has been adapted on a commercial cryomicrotome and provides a powerful modality for ex vivo imaging of small animals. Results: The ability to capture 3D anatomical (color) and fluorescence volumetric distributions of multiple fluorescent markers in high resolution is showcased. Conclusions: Serving both as a method for accurately imaging the bio-distribution of multiple fluorescent agents inside organisms and as a modality for the validation of non-invasive methods, multispectral cryoslicing imaging offers useful insights to ex vivo optical imaging of molecular probes.
Altmetric
Weitere Metriken?
Zusatzinfos bearbeiten
[➜Einloggen]
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Optical imaging; Fluorescence; Multispectral imaging; Three-dimensional imaging
ISSN (print) / ISBN
1536-1632
e-ISSN
1860-2002
Zeitschrift
Molecular Imaging and Biology
Quellenangaben
Band: 13,
Heft: 5,
Seiten: 874-885
Verlag
Elsevier
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed