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Ebrahim, A.M.* ; Alnajjar, A.O.* ; Mohammed, M.E.* ; Idris, A.M.* ; Mohammed, M.E.A.* ; Michalke, B.

Investigation of total zinc contents and zinc-protein profile in medicinal plants traditionally used for diabetes treatment.

Biometals 33, 65-74 (2020)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
This study aims at the assessment of total zinc contents, water zinc extract contents and zinc-protein profile in medicinal plants traditionally used for diabetes treatment. While zinc-protein profile was screened in plant samples using the online coupling of size exclusion chromatography-inductively coupled plasma-mass spectrometry (SEC-ICP-MS), total zinc contents and zinc water extract contents were determined using inductively coupled plasma-optical emission spectrometry (ICP-OES). The analysis of two certified reference materials with similar matrix for total zinc content revealed recovery values of 97.1% and 100.5% while the average of recovery of the summed Zn concentrations from protein fractions compared to total Zn was 103.0 +/- 4.8%. Based on the FAO/WHO classification, Sesamum indicum, Nigella sativa, Trigonella Foenum-graecum and Pennisetum glaucum are classified as highest Zn-content. For protein profile, zinc was quantified in 330-430 and 50-60 kDa fractions of all examined plants while no contents were quantified in the inorganic fraction 0.05-0.4 kDa of all plant species. Also, only three plant species recorded Zn contents in the phytate fraction (0.9-1.5 kDa fraction). The fruits of the Momordica Charantia and the Citrullus colocynthis were with the highest extractable zinc concentration; 13.55 +/- 0.45 and 10.08 +/- 0.63 mg/kg, respectively. The highest Zinc capturing capacity was reported for the 50-60 and 70-87 KDa protein fractions.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Diabetes ; Sec-icp-ms ; Trace Element Speciation ; Zinc; Serum Trace-elements; Plasma-mass Spectrometry; Momordica-charantia; Antidiabetic Activities; Speciation Analysis; Saudi-arabia; Heavy-metals; Bitter-melon; Fruit; Fish
ISSN (print) / ISBN 0966-0844
e-ISSN 1572-8773
Zeitschrift BioMetals
Quellenangaben Band: 33, Heft: 1, Seiten: 65-74 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Analytical BioGeoChemistry (BGC)