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D'Arienzo, V.* ; Magri, A.* ; Harris, J.M.* ; Wing, P.A.C.* ; Ko, C. ; Rubio, C.O.* ; Revill, P.A.* ; Protzer, U. ; Balfe, P.* ; McKeating, J.A.*

A PCR assay to quantify patterns of HBV transcription.

J. Gen. Virol. 102:001373 (2019)
Verlagsversion DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
Hepatitis B virus (HBV) is the prototype member of the family Hepadnaviridae and replicates via episomal copies of a covalently closed circular DNA (cccDNA) genome of approximately 3.2 kb. The chromatinization of this small viral genome, with overlapping open reading frames and regulatory elements, suggests an important role for epigenetic pathways to regulate HBV transcription. However, the host pathways that regulate HBV transcription and the temporal nature of promoter usage in infected cells are not well understood, in part due to the compact genome structure and overlapping open reading frames. To address this we developed a simple and cost-effective PCR assay to quantify the major viral RNAs and validated this technique using current state-of-art de novo HBV infection model systems. Our PCR method is three orders of magnitude more sensitive than Northern blot and requires relatively small amounts of starting material, making this an attractive tool for assessing HBV transcription.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Hepatitis B Virus ; Rna ; Transcription
Sprache englisch
Veröffentlichungsjahr 2019
HGF-Berichtsjahr 2019
ISSN (print) / ISBN 0022-1317
e-ISSN 1465-2099
Zeitschrift Journal of General Virology
Quellenangaben Band: 102, Heft: 3, Seiten: , Artikelnummer: 001373 Supplement: ,
Verlag Society for General Microbiology
Verlagsort Charles Darwin House, 12 Roger St, London Wc1n 2ju, Erks, England
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Virology (VIRO)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) G-502700-003
Förderungen Wellcome Trust
MRC
EU 2020 Research and Innovation Programme Consortia HEP-CAR
DOI 10.1099/jgv.0.001373
WOS ID WOS:000635958900002
PubMed ID 31846416
Erfassungsdatum 2020-01-15
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