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Ueda, H.R.* ; Ertürk, A. ; Chung, K.* ; Gradinaru, V.* ; Chedotal, A.* ; Tomancak, P.* ; Keller, P.J.*

Tissue clearing and its applications in neuroscience.

Nat. Rev. Neurosci. 21, 61-79 (2020)
Postprint DOI PMC
Open Access Green
State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact tissues from individual organs and even to some entire mammals. When combined with light-sheet microscopy and automated approaches to image analysis, existing tissue-clearing methods can speed up and may reduce the cost of conventional histology by several orders of magnitude. In addition, tissue-clearing chemistry allows whole-organ antibody labelling, which can be applied even to thick human tissues. By combining the most powerful labelling, clearing, imaging and data-analysis tools, scientists are extracting structural and functional cellular and subcellular information on complex mammalian bodies and large human specimens at an accelerated pace. The rapid generation of terabyte-scale imaging data furthermore creates a high demand for efficient computational approaches that tackle challenges in large-scale data analysis and management. In this Review, we discuss how tissue-clearing methods could provide an unbiased, system-level view of mammalian bodies and human specimens and discuss future opportunities for the use of these methods in human neuroscience.Tissue-clearing methods are now allowing 3D imaging of intact tissues and some entire mammals. In this Review, Ueda and colleagues discuss the various tissue-clearing methods, related techniques and data analysis and management, as well as the application of these methods in neuroscience.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Review
Korrespondenzautor
Schlagwörter Single-cell Resolution; Light-sheet Microscopy; Optical Coherence Tomography; Whole-body; Molecular Interrogation; Cancer Metastasis; Brain Activity; Intact-tissue; Stem-cells; Long-term
ISSN (print) / ISBN 1471-003X
e-ISSN 1471-0048
Zeitschrift Nature Reviews - Neuroscience
Quellenangaben Band: 21, Heft: 2, Seiten: 61-79 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort Macmillan Building, 4 Crinan St, London N1 9xw, England
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Tissue Engineering and Regenerative Medicine (ITERM)